An overview of an experiment on the cell membrane permeability

Overview, and fundamental considerations for stable and reproducible measurements Dec 24, Introduction In vivo, barriers are provided by monolayers of epithelial or endothelial cells. In many diseases as well as in inflammation, these barriers become compromised, and hence, measuring their permeability is of considerable interest to cell biologists and the health community in general.

An overview of an experiment on the cell membrane permeability

Cell Membrane Structure Investigating the effect of temperature on plant cell membranes Class practical You and your students may be familiar with the observation that colour leaks out of beetroot when it is cooked.
Botany : Lecture 5 - Cell membrane: Properties and Selective Permeability Lecture 5 - Cell membrane: Properties and Selective Permeability Lesson Developer:

Many factors affect how this membrane behaves and temperature is one of the most important. Temperature helps determine what can enter or leave the cell and how well molecules found within the membrane can function. Temperatures that are too high or too low can seriously damage and, in the extreme temperature ranges, kill the cell through their effect on the cell's membrane.

What Makes a Cell Membrane? A cell membrane is called a bilayer because it's made of two layers that face each other and surround the cell. Chemically, each layer is formed by fatty molecules called phospholipids. Each molecule has an end that repels water, called its head, and another end called the tail that repels water.

The nature of the phospholipids in the membrane helps keep it fluid and semi-permeable, so that some molecules like oxygen, carbon dioxide and small hydrocarbons can move through it and enter the cell, while other molecules that might be harmful or unneeded by the cell are kept out.

Why Does Cholesterol Lower Membrane Permeability? | Healthfully

A cell membrane also contains proteins, either on its inner or outer surface — called peripheral proteins — or embedded in the membrane and called integral proteins. Because the membrane is fluid and not rigid, these proteins can move within the membrane to serve the cell's needs and help keep it healthy.

An overview of an experiment on the cell membrane permeability

Also, as cells grow and enlarge, the membrane also increases in size and maintains its fluidity to allow this growth to proceed smoothly. If body temperature increases, for example during a high fever, the cell membrane can become more fluid.

This happens when the fatty acid tails of the phospholipids become less rigid and allow more movement of proteins and other molecules in and through the membrane. This can change the permeability of the cell, possibly allowing some potentially harmful molecules to enter.

Both integral and peripheral proteins in the membrane can also be damaged by high temperatures and, if extremely high, heat might cause these proteins to break down, or denature.

Low Temperature Stiffens the Membrane A decrease in temperature can also have a negative effect on cell membranes and cells. At low temperature, the fatty acid tails of the phospholipids move less and become more rigid.

This decreases the overall fluidity of the membrane, also decreasing its permeability and potentially restricting entry of important molecules such as oxygen and glucose into the cell.

Low temperature can also slow cell growth by prevent the cell's increase in size. In extreme situations, such as prolonged exposure to sub-freezing temperatures, liquid in the cell can begin to freeze, forming crystals that pierce the membrane and might ultimately kill the cell.

An overview of an experiment on the cell membrane permeability

Measures of Membrane Fluidity About the Author Stephanie Chandler is a freelance writer whose master's degree in biomedical science and over 15 years experience in the scientific and pharmaceutical professions provide her with the knowledge to contribute to health topics.

Chandler has been writing for corporations and small businesses since In addition to writing scientific papers and procedures, her articles are published on Overstock.Effect Of Ethanol On Beetroot Membrane. Biology Coursework Hypothesis: To investigate the effect of different concentrations of ethanol on the permeability of beetroot cell membranes.

Prediction: By exposing a membrane to a solvent, ethanol, it will increase its permeability. and permeability of the cell membrane depends on its intact structure.

When destroyed, the permeability of the cell membrane is disrupted causing cellular contents to leak out. The cell membrane can be destroyed by physical damage, chemicals and high temperature. High. A typical A Level membrane permeability experiment involves investigating the influence of a named variable on the membrane permeability of a vegetable such as beetroot (Beta vulgaris).

Common variables to investigate are the effect of solvents or temperature because both of these factors can change the fluidity of the membrane.

Lecture 5 - Cell membrane: Properties and Selective Permeability Botany Notes | EduRev

There are factors that affect the fluidity of the cell membrane, and these can affect membrane permeability making it easier for us to influence what substances can . A cell membrane is called a bilayer because it's made of two layers that face each other and surround the cell.

Chemically, each layer is formed by fatty molecules called phospholipids. Each molecule has an end that repels water, called its head, and another end called the tail that repels water.

Cell Membrane Permeability and Osmosis Experiment 3 Objectives: To demonstrate the mechanism involved in Osmosis; To demonstrate the tonicity of solutions by subjecting the cells to different concentration of solute.

Fluid mosaic model: cell membranes article (article) | Khan Academy